Resurrected demon girl who creates Mayuka to manipulate Tenchi. Takes Sasami hostage, erases Mayuka's memories, and transforms into a giant dark creature before being defeated by Tenchi.
Yuzuha artificially creates Mayuka using genetic material for her own amusement.
The primary antagonist whose actions drive the entire conflict against the protagonist.
Yuzuha captures Sasami to force the heroes into a disadvantageous battle.
Yuzuha's forces directly harm Ryoko, cementing their adversarial relationship.